Network Pharmacology and Molecular Docking Integrated Strategy to the Screening of Active Components and Mechanisms of Stephaniae Tetrandrae Radix on Breast Cancer

Stephaniae Tetrandrae Radix (STR) is a commonly used herb with history of thousands years. Accumulating evidence shows the therapeutic effect on breast cancer (BC) STR. Here, we aimed to elucidate active components and mechanisms STR against BC. The targets were retrieved screened from corresponding databases. A target protein–protein interaction (PPI) network was built Ingenuity Pathway Analysis (IPA) analyze screen key pathways. Subsequently, molecular docking performed visualize patterns interactions between targets. Finally, main in treating BC confirmed by vitro experiments, 34 common obtained. PPI IPA showed that TP53, JUN, CASP3, so on. Additionally, signaling pathways enriched. Docking verified have good binding potential targets, especially tetrandrine (Tet) fangchinoline (Fang). In studies they significantly inhibited viability MDA-MB-231 cells increased LDH leakage rate compared MCF-10A cells. participates many cell processes regulate multiple thereby playing an anti-breast role. Tet Fang may be components.